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mouse anti wg  (Developmental Studies Hybridoma Bank)


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    Structured Review

    Developmental Studies Hybridoma Bank mouse anti wg
    Mouse Anti Wg, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 96/100, based on 147 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+wg+antibody/anti-Wingless+protein/pm41888616-50-8-12
    Average 96 stars, based on 147 article reviews
    mouse anti wg - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Mutagenesis:

    Article Title: Migrating cells control morphogenesis of substratum serving as track to promote directional movement of the collective
    Article Snippet: The primary antibodies used in this study were goat anti-GFP (1:5000; Rockland, 600-103-215), rabbit anti-RFP (1:1000; MBL International, PM005), mouse anti-FasIII [1:200; Developmental Studies Hybridoma Bank (DSHB), 7G10], mouse anti-βPS1 (1:40; DSHB, CF.6G11), guinea pig anti-Htl (1:1000; Sun and Stathopoulos, 2018 ), rat anti-Twi (1:200; Ozdemir et al., 2011 ), rabbit anti-Zfh1 (1:2000; gift from R. Lehmann; Broihier et al., 1998 ), guinea pig anti-Hb9 (1:1000; gift from J. Skeath; Broihier and Skeath, 2002 ), rabbit anti-phospho-FAK (1:1000; Thermo Fisher Scientific, 44-624G) and rabbit anti-Tey (1:500; this study). .. Anti-Wg antibody (1:10; DSHB, 4D4) was used to determine embryo stage to compare wild-type and HLH54F mutant embryos. .. Chromogenic staining was performed using the Vectastain Elite ABC system in conjunction with DAB substrate for signal development (Vector Laboratories).

    other:

    Article Title: Migrating cells control morphogenesis of substratum serving as track to promote directional movement of the collective
    Article Snippet: Anti-Tey antibodies were affinity purified from the resulting serum by conjugating the Tey protein fragment above to beads following the manufacturer's protocol (Aminolink coupling resin, Thermo Fisher Scientific).

    Control:

    Article Title: Wing patterning in faster developing Drosophila is associated with high ecdysone titer and wingless expression.
    Article Snippet: Supernatants were transferred to fresh tubes and quantified using BCA kit reagent (Thermo-scientific). .. An equal amount of protein extract from the control and selected populations was run on SDS-PAGE at 50 V for 30 min (stacking) followed by 100 V for 180 min. Gel was transferred to nitrocellulose membrane (Bio-Rad) using Mini Trans-Blot cell apparatus (Bio-Rad) for 135 min at 50 V. For Western blotting, the membrane was incubated with TBS containing skimmed milk for 60 min followed by overnight incubation at 4 °C with anti-Wg antibody (#4D4, 1:100) from DSHB. .. Next day membrane was washed thrice with TBS containing 0.01% of Tween-20 followed by incubation with secondary anti-mouse HRP (Sigma, 1:1000) for 60 min at RT.

    SDS Page:

    Article Title: Wing patterning in faster developing Drosophila is associated with high ecdysone titer and wingless expression.
    Article Snippet: Supernatants were transferred to fresh tubes and quantified using BCA kit reagent (Thermo-scientific). .. An equal amount of protein extract from the control and selected populations was run on SDS-PAGE at 50 V for 30 min (stacking) followed by 100 V for 180 min. Gel was transferred to nitrocellulose membrane (Bio-Rad) using Mini Trans-Blot cell apparatus (Bio-Rad) for 135 min at 50 V. For Western blotting, the membrane was incubated with TBS containing skimmed milk for 60 min followed by overnight incubation at 4 °C with anti-Wg antibody (#4D4, 1:100) from DSHB. .. Next day membrane was washed thrice with TBS containing 0.01% of Tween-20 followed by incubation with secondary anti-mouse HRP (Sigma, 1:1000) for 60 min at RT.

    Membrane:

    Article Title: Wing patterning in faster developing Drosophila is associated with high ecdysone titer and wingless expression.
    Article Snippet: Supernatants were transferred to fresh tubes and quantified using BCA kit reagent (Thermo-scientific). .. An equal amount of protein extract from the control and selected populations was run on SDS-PAGE at 50 V for 30 min (stacking) followed by 100 V for 180 min. Gel was transferred to nitrocellulose membrane (Bio-Rad) using Mini Trans-Blot cell apparatus (Bio-Rad) for 135 min at 50 V. For Western blotting, the membrane was incubated with TBS containing skimmed milk for 60 min followed by overnight incubation at 4 °C with anti-Wg antibody (#4D4, 1:100) from DSHB. .. Next day membrane was washed thrice with TBS containing 0.01% of Tween-20 followed by incubation with secondary anti-mouse HRP (Sigma, 1:1000) for 60 min at RT.

    Western Blot:

    Article Title: Wing patterning in faster developing Drosophila is associated with high ecdysone titer and wingless expression.
    Article Snippet: Supernatants were transferred to fresh tubes and quantified using BCA kit reagent (Thermo-scientific). .. An equal amount of protein extract from the control and selected populations was run on SDS-PAGE at 50 V for 30 min (stacking) followed by 100 V for 180 min. Gel was transferred to nitrocellulose membrane (Bio-Rad) using Mini Trans-Blot cell apparatus (Bio-Rad) for 135 min at 50 V. For Western blotting, the membrane was incubated with TBS containing skimmed milk for 60 min followed by overnight incubation at 4 °C with anti-Wg antibody (#4D4, 1:100) from DSHB. .. Next day membrane was washed thrice with TBS containing 0.01% of Tween-20 followed by incubation with secondary anti-mouse HRP (Sigma, 1:1000) for 60 min at RT.

    Incubation:

    Article Title: Wing patterning in faster developing Drosophila is associated with high ecdysone titer and wingless expression.
    Article Snippet: Supernatants were transferred to fresh tubes and quantified using BCA kit reagent (Thermo-scientific). .. An equal amount of protein extract from the control and selected populations was run on SDS-PAGE at 50 V for 30 min (stacking) followed by 100 V for 180 min. Gel was transferred to nitrocellulose membrane (Bio-Rad) using Mini Trans-Blot cell apparatus (Bio-Rad) for 135 min at 50 V. For Western blotting, the membrane was incubated with TBS containing skimmed milk for 60 min followed by overnight incubation at 4 °C with anti-Wg antibody (#4D4, 1:100) from DSHB. .. Next day membrane was washed thrice with TBS containing 0.01% of Tween-20 followed by incubation with secondary anti-mouse HRP (Sigma, 1:1000) for 60 min at RT.



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